INHIBITION OF LIPOXYGENASE BY ELEPHANTOPUS SCABER EXTRACT AND DETERMINATION OF ITS INHIBITION PATTERN
Elephantopus scaber Linn, a medicinal herb indigenous to India, has been widely used in treating a number of inflammatory pathological conditions. In the present study, methanolic extract of E. scaber was evaluated for its ability to inhibit 5, 12 and 15 Lipoxygenase enzymes (LO) and the nature of inhibition was determined from Km and Vmax values obtained from Lineweaver Burk plot. The methanolic extract was found to inhibit all three LO enzymes in a mixed competitive non competitive nature. E. scaber promises to be a good source of bioactive molecules that could be employed for inflammation research.
MICROBIAL SPOILAGE OF CANNED TOMATO SOLD IN BENIN CITY
Tomato (solanum lycopersicum), is one of the most important vegetable crop of the solanaceae family, grown all over the world for food and other economic purposes. The microbial spoilage of canned tomato of varying product brands was investigated in this study. It was observed that the total aerobic and anaerobic counts were less than 103 cells which were within acceptable limit. Two of the canned product had no microbial count, while the others had counts varying from 2 x 101 to 5 x 101 for aerobic count of freshly opened tomato, and 1 x 101 to 2 x 101 for anaerobic count. However, aerobic count of spoilt canned tomato product ranged from 4.2 x 104 to 9.1 x 104 across all six products investigated. Anaerobic count of spoilt canned tomato ranged from 2.5 x 104 to 6.8 x 104. Isolated organisms obtained from spoilt canned tomato samples revealed the presence of Bacillus ploymyxa, Staphylococcus aureus, Streptococcus lactis, Pseudomonas, sp., Clostridium sporogenes, Bacillus coagulans, Saccharomyces sp., Candida sp., Mucor sp., Aspergillus niger, and Penicillium sp. These organisms were implicated in the spoilage of canned tomato products sold in Benin City. When these products are consumed immediately after opening, they are safe because the microbial load on fresh product is within acceptable regulatory standard.
BREAST FEEDING PRACTICES AMONG POSTNATAL MOTHERS- A HOSPITAL BASED STUDY
To assess the knowledge and practices of mothers about breastfeeding Practices Cross-sectional study was carried out for a period of one month. A total of 162 postnatal mothers admitted in postnatal ward of OBG dept in Shri.B M. Patil medical college constitute the sample size. The data was collected using semi-structured questionnaires. Data was analyzed by using SPSS 16th version. The study shows that 21(12.9%) of the babies were feed with prelacteal feeds like honey and sugar water. The significant association was found between prelacteal feeds versus education and socio-economic status. The study concludes that the health care staff of postnatal ward must utilize the opportunity to persuade mothers for timely initiation of breastfeeding practices.
SYNTHESIS OF SUBSTITUTED 5-PHENYL-1-(5-PHENYL)-ISOXAZOL-3-YL)-1H-TETRAZOLE AS ANTIOXIDANT AGENTS
A new series of substituted 5-phenyl-1-(5-phenyl)-isoxazol-3-yl)-1H-tetrazole have been synthesized from the reaction of sodium azide, benzonitrile and ammonium chloride followed by acetylation. The resulted intermediate was reacted with substituted aromatic aldehyde to yield substituted 1-(5-phenyl-1H-tetrazol-1-yl) prop-2-en-1-one which upon cyclization with hydroxylamine hydrochloride form titled compounds. The structures of synthesized compounds were characterized by IR, 1H NMR and mass spectral data. All the synthesized compounds were investigated for their in-vitro antioxidant activity by scavenging of DPPH free radical. The result of antioxidant activity revealed that the compounds 21 and 23 have shown potent activity.
A BAYESIAN APROACH FOR ZERO-INFLATED COUNT DATA IN PSYCHOLOGICAL RESEARCH
Count data in psychological research are commonly modelled using zero-inflated Poisson regression. This model can be viewed as a latent mixture of an “always-zero” component and a Poisson component. In this study we introduce a Bayesian approach for zero inflated Poisson model, and discuss model comparisons and the interpretation of their parameters. As illustrated with two real-world examples psychological research, both Bayesian and classic approach of models can easily be fitted with a great gain.
EVALUATION OF PHYTOCONSTITUENTS OF METHANOLIC ROOT EXTRACT OF WITHANIA SOMNIFERA
The present research work was designed to identify the phytochemical constituents of methanolic extract of roots of Withania somnifera. Withania somnifera is widely used in Ayurvedic medicine, the traditional medical system of India. It is a member of the family Solanaceae. Therapeutic importance of the different parts of this plant has a long history. Fresh mature roots were shade dried at room temperature, coarse powdered and extracted with methanol by Soxhlet’s extraction method. Thereafter, the extract was concentrated using rotary flash evaporator to obtain semisolid crude extract with the yield of 10.682%. Withania somnifera root extract was investigated for the presence of phytochemical constituents. The preliminary phytochemical evaluation of the Withania somnifera root extract revealed the presence of alkaloids, flavonoids, phenolics, carbohydrates, tannins, steroids and starch.