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Sciensage

2014-Vol 5-Issue 3

Research Articles

POROUS TINI-BASED ALLOY SCAFFOLD FOR CELL TISSUE ENGINEERING

Recently, a new fabrication method of scaffolds based on porous TiNi alloy has been developed for tissue engineering. This provides significant advantages over conventional scaffolds, including: versatility, reproducibility and industrial scalability. Structural properties of porous TiNi-based scaffolds have been investigated. The microporous surface of walls was shown to create the condition for cells adhesion and ingrowth, it facilitates the penetration of nutrients from outside and metabolite’s outflow during the study. Features of cell growth, which makes different tissue populations and step-by-step cellular interaction with the porous- permeable structure of TiNi-based scaffold were established. Methods used in this study proved the tissue ingrowth within scaffold’s pores. Cells implanted to be well protected from the allogenic environment were comprehensively found in vitro to fill all the pore space and to create specific tissues.

Kokorev O.V., Hodorenko V.N., Chekalkin T.L., Dambaev G.Ts., Gunther V.E.

Tomsk State University, Research Institute of Medical Materials, (Tomsk, Russia)

BIOLOGICAL POTENTIALS OF EXTRACTS AND COMPOUNDS FROM MAMMEA USAMBARENSIS VERDC FRUIT

In attempt to investigate some biological activities of Mammea usambarensis fruit, absolute ethanolic crude extract, 80% DCM/PE fraction, 5% MeOH/DCM fraction and mammea B/AB were subjected to toxicity test in brine shrimps and mice, antimicrobial activity by disc diffusion and broth dilution method and antioxidant activity by DPPH scavenging activity. In the brine shrimps toxicity test, the crude extract, 80% DCM/PE fraction, 5% MeOH/DCM fraction and mammea B/AB were highly toxic with LC50 values of 1.58, 1.14, 3.89 and 5.21 µg/ml, respectively.  The crude extract was non-toxic to mice even at a dose above 2000 mg/kg body weight. The crude extract, 80% DCM/PE 5% MeOH/DCM fractions and mammea B/AB exhibited low activity with zone of inhibition of 8.3-16 mm for Salmonella kisarawe, 8.7-14 mm for Staphylococcus aureus and 8.3-10 mm for Klebsiella oxytoca. The minimum inhibitory concentrations ranged from 3.75 µg/µl-25 µg/µl. All samples tested had no activity against Candida albicans and Cryptococcus neoformans. In DPPH assay crude extract, 80% DCM/PE fraction, 5% MeOH/DCM fraction and mammea B/AB had lower scavenging activity of 86, 69, 72 and 77 µg/ml: mM, respectively, compared to the standard compound, propagyl gallate, which had a value of 93 µg/mL: mM. To conclude, the antioxidant activity exhibited prompt further studies aimed at discovery of antioxidant agents.

Pax J. Masimba, Joseph J. Magadula, Zaituni Msengwa, Rose B. Tarimo, Zakariah H. Mbwambo, Matthias Heydenreich, Dimitri Breard, Pascal Richomme , Eltayeb Fadul Fadul Alla

Institute of Traditional Medicine, Muhimbili University of Health and Allied Sciences, P.O. Box 65001, Dar es Salaam, Tanzania.

ISOLATION AND IDENTIFICATION OF ANTI-ESBL (EXTENDED SPECTRUM β-LACTAMASE) COMPOUND FROM MARINE STREPTOMYCES SP. VITSJK8

Multidrug resistant extended spectrum β-lactamse producing organisms are more frequently reported from urinary tract infected UTI patients. Recent reports have shown that ESBL’s efficiently hydrolyze third generation cephalosporins and monobactams, thereby limiting the therapeutic options. The aim of the present study was to isolate and to identify anti-ESBL compound from marine Streptomyces sp.VITSJK8. It was isolated from marine sediment sample collected at Cheyyur beach, Bay of Bengal, Tamil Nadu, India. The ethyl acetate extract from the culture supernatant of Streptomyces sp.VITSJK8 was prepared and purified by silica gel column chromatography and HPLC. The purified compound was identified by using 1H and 13C NMR spectral data and based on the similarity index with reference compounds available in the mass spectra library of National Institute for Standards and Technology as 1, 2-benzene dicarboxylic acid, mono (2-ethylhexyl) ester. The anti-ESBL activity of the purified compound was tested against ESBL isolates according to the National Committee for Clinical Laboratory Standards guidelines and it was revealed that its MIC50 value ranged from 0.13 to 2.00 µg/mL. The results of the study suggest that Streptomyces sp.VITSJK8 is the potential source for anti-ESBL compounds.

Esther Subashini, Krishnan Kannabiran

School of Biosciences and Technology, VIT University, Vellore, India

CORRELATION OF THE PERSONALITY TRAITS WITH THE REASONS FOR DIVORCE IN I.T. PROFESSIONALS

The present study aimed to examine the relationship between the personality traits and certain domains of reasons for divorce. A total of 412 litigants (those who have applied for divorce) in family courts of Chennai have been interviewed personally by the investigator. Among them all 412 had revealed the reasons for divorce and only 399 had consented for personality traits assessment. A non probability convenient sampling technique was adopted to select the samples. Spearman rank order correlation was used to analyze the data. Results revealed that there is positive correlation between illness reason with conscientiousness traits at p= 0.0195 and r= 0.118.Findings showed that there is relationship between the personality traits and certain domains of reasons for divorce. 

P.Subha Rani

Saveetha College of Nursing, Saveetha University, Thandalam, Chennai, India

SCREENING OF CELLULASE PRODUCING MICROORGANISMS FROM LAKE AREA CONTAINING WATER HYACINTH FOR ENZYMATIC HYDROLYSIS OF CELLULOSE

Microorganisms are capable to hydrolysis of cellulose, only a few of these produce significant quantities of cell free enzyme capable of completely hydrolyzing crystalline cellulose in-vitro. Fungi are the main cellulase producing microorganisms, through a few bacteria and actinomycetes have also been reported to yield cellulase activity like Trichoderma Sp., Aspergillus Sp., Pseudomonas Sp., Cellulomonas Sp., Bacillus Sp., and Micrococcus Sp. Most of the aquatic systems are infested with fast growing rate and high capacity for survival Eichhornia crassipes (water hyacinth). Plants (water hyacinth), water and soil collected from Coimbatore lakes and isolated thirty six species of microorganisms have been isolated, which were used for the enzyme (cellulase, xylanase) production. Ten fungi (F), eight bacteria (B), twelve yeasts (Y) and six actinomycetes (A) were used for degradation of cellulose and xylan for bio-fuel production. The cellulose and xylan activity of the organisms was measured diameter of a clear zone around the colony and hydrolytic value on cellulose Congo red agar media. Growth of organisms is very effective at temperature 28 ± 2 ˚C and pH 6.0 ± 0.4 in aerobic condition and 2 to 5 days of the incubation period.

Sudarshan Singh Rathore, Mannivannan A., R. T. Narendhirakannan

Department of Biotechnology, School of Biotechnology and Health sciences, Karunya University (Karunya Institute of Technology and Science), Karunya Nagar, Coimbatore, Tamil Nadu, India

IMMUNIZATION STATUS AND OCCURENCE OF ACUTE RESPIRATORY INFECTION IN UNDER FIVE CHILDREN

Background: Pneumonia is the leading cause of morbidity and mortality in children below five years of age. Immunization against certain diseases like measles, diphtheria, pertusis, and with BCG may protect the child against LRI. Objectives: To study the prevalence of ARI among under five children residing in the urban slum and the effect of immunization on the occurrence of ARI in the study population. Method: Cross Sectional observational study carried out in the urban slum, Raichur. Results: Out of 451 children enrolled in the study, 75 children had ARI at the time of survey; hence the prevalence rate is 16.6%. Completely   immunized children were 67.4% and 32.6% were partially immunized. Conclusion: A significantly higher prevalence was found for age. Partially immunized children had higher prevalence. Improving the immunization status will help to reduce the burden of ARI.

Mallikarjun K Biradar, Santosh M Biradar

Dept of Community Medicine, BLDE University Shri B.M.Patil medical college, Bijapur. India

DEVELOPMENT AND VALIDATION OF ANALYTICAL METHOD FOR SIMULTANEOUS ESTIMATION OF CILNIDIPINE AND OLMESARTAN MEDOXOMIL IN BULK AND TABLET DOSAGE FORM BY HPTLC

Cilnidipine and Olmesartan medoxomil in combined dosage form has been developed and validated. Sample and standard solutions of Cilnidipine and Olmesartan medoxomil were applied to precoated silica gel G 60 F254 HPTLC plates and the plates were developed with Methanol: Toluene: Ethyl acetate: Acetic  acid  in the ratio 2.5: 5.5: 2.0: 0.1 (v/v/v/v) as mobile phase. The Rf value for cilnidipine and Olmesartan medoxomil was found to be 0.66 and 0.43 respectively The detection was performed at 254 nm. The calibration curve was found to be linear between 100 to 200 ng/spot for cilnidipine and 200 to 400 ng/spot for Olmesartan medoxomil with correlation coefficients 0.995 and 0.996 for Cilnidipine and Olmesartan medoxomil respectively. The LOD and LOQ were found to be 23.057ng/spot and 69.86 ng/spot for Cilnidipine and 39.18ng/spot and 118.750 ng/spot for Olmesartan medoxomil respectively. The results have been validated statistically as per ICH guidelines.

Amit S. Minase*, Manjusha N. Dole

Department of Pharmaceutical Chemistry, Smt. Kashibai Navale College of Pharmacy, Kondhwa, Pune, Maharashtra, India

DEVELOPMENT AND VALIDATION OF STABILITY-INDICATING RP-HPLC METHOD FOR ESTIMATION OF DABIGATRAN ETEXILATE

A simple, selective and rapid stability indicating reverse phase high performance liquid chromatographic (RP-HPLC) method for the estimation of Dabigatran Etexilate has been developed and validated. Analyte was resolved on a Neosphere C8 (150mm X 4.6 mm) column. The mobile phase consisted of Methanol: Phosphate Buffer (0.01M pH 3)   in the ratio of 60:40 ʋ/ʋ and sonicated to degas. It was delivered at a flow rate of 1ml/min at ambient temperature and the retention time was about 4.4±0.05 minutes. Studies were performed on an HPLC system equipped with a PDA detector at 225nm. The drug was subjected to stress condition of hydrolysis (acid, base, neutral), oxidation, photolysis and thermal degradation. The calibration curve was linear over the concentration range of 1-5μg/ml (R=0.999). The limit of detection for Dabigatran Etexilate was found to be 0.014 μg/ml and the quantification limit was about 0.040 μg/ml. The accuracy of the method was established based on the recovery studies. The proposed method is applicable to the routine analysis of Dabigatran Etexilate.

Mrinalini C.Damle, Rupesh A. Bagwe

Department of Quality Assurance, AISSMS College of Pharmacy, Pune, Maharashtra, India

Short Communications

ASSESMENT OF HYDRO-GEOCHEMICAL PARAMETER AND CORRELATION MATRIX OF GROUND WATER AT PIRBAWDA, AURANGABAD (M.S.)

The ground water samples were collected from Pirbawda station at Aurangabad district was evaluated during 2009-10for its hydro- geochemical parameters regularly in every month. A systematic study of correlations among the water quality parameters has been carried out. The parameters studied were pH, Conductivity, TDS, Turbidity, Hardness, Ca, Mg, Na, K, Fe, Alkalinity, CO3, HCO3, Cl, F, NO3 and SO4. Monthly variation in the parameters was discussed here. All parameters were found to be within permissible limit.

Rashmi Pathrikar, Megha Rai, Mohammad Mohsin and B.R.Agarwal

P.G. & Research Centre, Maulana Azad College, Aurangabad (M.S.), India


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