The present investigation was carried out to determine the Lethality, Injury, Pollen grain sterility, Effectiveness and Efficiency induced by EMS (Ethyl methane sulphonate) in the concentration 20mM,30mM,40mM and DES (Diethyl sulphate) in the concentration 30mM, 40mM, 50mM. The M2 generation seeds were subjected to observe the mutation frequency which acts as an inducing factor for these variations. The Lethality was found to be increased with increase in the concentration. In EMS the Maximum Effectiveness was observed in the low concentration of 20mM (0.35%) and in DES in the concentration of 30mM (0.24%) and the Efficiency was observed to be maximum in the concentration 20mM (44.76%) EMS and (39.92%) in 30mM of DES. EMS was more effective and efficient in stimulating variability in fox tail millet than DES. Therefore the mutants of Setaria italica var. Co (Te) 7 with less biological damage and high mutation frequency can be developed at low concentration of EMS and DES can be chosen for plant breeding in Fox tail millet.
Journal of Advanced Scientific Research
I. Anittha*, L. Mullainathan
Department of Botany, Annamalai University, Annamalai Nagar 608002, Tamil Nadu, India