BIOCHEMICAL CHARACTERIZATION AND ANTIBIOTIC SENSITIVITY PROFILING OF UROPATHOGENIC BACTERIAL STRAINS ISOLATED FROM URINE SAMPLE OF UTI PATIENTS

Abstract

Prevalence of Urinary tract infections (UTIs) holds the second position among the various infectious disease caused by uropathogenes. Abuse and improper prescribing policy of antibiotics induces multidrug resistance property among the causative agents of Urinary Tract Infections (UTIs).The main intend of this study was to isolate, biochemically characterize and to monitor the antibiotic susceptibility property among uropathogenic bacterial strains from urine samples of UTIs positive outdoor patients. A total number of twenty five (25) UTI patient’s left over urine samples were collected from the various pathological laboratories at Malda Town among them twenty two (22) gave significant culture growth responses. Fifty two (52) bacterial strains were isolated from the twenty two (22) urine samples, whereas 42.30% were gram positive and 57.69% were gram negative strains. Characterization of isolated strains was determined by using different selective media and various biochemical assays such as, IMViC, Oxidase, gelatine hydrolysis, motility and urease assays etc. The maximum numbers of isolated bacterial strains were Escherichia coli and Staphylococcus aureus. In this study, 83.33% of gram negative bacteria were indole positive and 96% of indole positive bacterial isolates were MR positive and 100% of indole positive bacterial strains were VP negative. Entire uropathogenic isolates were showed the positive results in the nitrate reduction assay. Antibiotic sensitivity profile suggested that, all the isolated strains were multi-drug resistant, among them a very few isolates were sensitive of the applied antibiotics. Thus, a strict regulatory approach should be applied to control such massive spread of antibiotic resistance.