Journal of Advanced Scientific Research

Exploring the Potential of Dyadobacter fermentans JDP9 Biosurfactants an Antibacterial and Antifouling Biocompatible Agent

2024-12-19T09:53:49+00:00

This study investigated the antibacterial and antiadhesive properties of a biosurfactant (BS) produced by Dyadobacter fermentans JDP9 against various Gram-positive and Gram-negative bacteria. The presence of BS was confirmed using oil spreading test, drop collapsing test and emulsification test. The BS demonstrated antimicrobial effects against Bacillus subtilis, Enterobacter aerogenes, Staphylococcus aureus, Pseudomonas aeruginosa, and Streptococcus faecalis at a 500 mg/mL concentration. Using a 96-well microtiter plate, the results indicated that both antibacterial and antiadhesive activities increased with higher BS concentrations. Glass slides coated with BS showed significantly reduced biofilm formation compared to control slides. The BS inhibited 71% of P. aeruginosa biofilm formation on a medical-grade catheter. Vero cells treated with BS exhibited moderate morphological changes with an IC₅₀ value of 217.5 μg/mL, indicating biocompatibility at 200 μg/mL. These findings suggest that BS, with its antibacterial, anti-adhesive, and biocompatible properties, holds potential as a biodegradable antifouling agent.

Hidden Potential of Aegle marmelos Fruit Peel

2024-12-19T09:53:49+00:00

Aegle marmelos or ‘Bael’ is a medicinal tree native to South Asia. The Bael fruit with the exception of the fruit peel, is extensively used in Ayurveda for its nutritive and healing properties. The present study aims to find the potential utilization and applications of the fruit peel by exploring its therapeutic value. Aegle marmelos fruit peel was subjected to extraction in methanol, ethanol, acetone and distilled water. Antimicrobial activity of the fruit peel extracts was tested against gram positive (B. subtilis, S. aureus) and gram negative (E. coli, K. pneumoniae) bacteria. MIC values were determined with strong evidence of broad-spectrum activity. DPPH assay revealed moderate, dose dependent antioxidant activity of the methanolic extract. Phytochemical screening showed the presence of flavonoids, alkaloids, tannins, phlobatannins, protein, cardiac glycosides and saponins. Nutritional and ash analysis of the fruit peel revealed presence of organic and inorganic compounds with dietary significance. GC-MS analysis of the fruit peel showed presence of various active phytoconstituents; notably ‘marmelosin’; a naturally present laxative agent in Aegle marmelos. Further research on the cytotoxic activity of the fruit peel maybe employed in the future, with superior extraction and purification strategies

Structural and Biological Studies of Newly Synthesized Co(II) Coordination Complexes with Hydrazine Hydrate-based Azomethine Ligands

2024-12-19T09:53:49+00:00

This research work reports the synthesis of novel biologically active cobalt-Azomethine coordination complexes [Co(L_n)₂Cl₂]. The Azomethine ligands have been synthesized by the condensation of hydrazine hydrate with aldehydes and ketones, which was then used to synthesize novel Co(II)-Azomethine coordination complexes and isolated as solid powdered product. The Azomethine ligands and their biologically active Co(II)-Azomethine coordination complexes [Co(L_n)₂Cl₂] were characterized for the molecular weight determination, elemental analysis, ¹H NMR and FT-IR spectroscopy. The Azomethine ligands and their Co(II)-Azomethine coordination complexes [Co(L_n)₂Cl₂] has also been characterized for the antimicrobial activity against several bacterial and fungal species, which has been associated with the standard bactericide Ciprofloxacin and fungicide Ketoconazole. The outcome of the research has indicated that Co(II)-Azomethine coordination complexes [Co(L_n)₂Cl₂] were found to retain excess antimicrobial activity against bacterial and fungal species as compared to the free azomethine ligands.