PROTECTIVE EFFECT OF HYDRO-ALCOHOLIC EXTRACT OF DRIED FRUITS OF HELICTERES ISORA IN ACETIC ACID INDUCED ULCERATIVE COLITIS IN EXPERIMENTAL RATS

Abstract

Ulcerative colitis is an inflammatory bowel disease which causes inflammation and ulceration in the lining of large
intestine i.e., colon. The aim of the present work was to study the effect of Hydro-alcoholic extract of dried fruits of
Helicteres isora in acetic acid induced ulcerative colitis in experimental rats. In current study wistar rats were randomly
divided in to five experimental groups. The control group received only saline by transrectal route. In second group i.e.,
negative control group experimental animals received only 2ml of 3% acetic acid for 14 days. Third group and fourth
group received low dose (100mg/kg) and High dose (200mg/kg) of hydroalcoholic extract of Helicteres isora dried fruits
respectively by oral route along with 2ml of 3% acetic acid for 14 days. Fifth group received prednisolone along with 2ml
3% acetic acid for 14 days. After treatment period rats of all groups were sacrificed on day 15th and their distal colonic
segments were dissect out, cut and assessed for macroscopic examination. The activity was evaluated in terms of lipid
peroxidation, superoxide dimutase (SOD) levels and Catalase (CAT) levels. The results of present study indicate
significant results of lipid peroxidation, superoxide dimutase and catalse concentrations. The present study also shows
significant results of C-reactive protein levels and histopathology of the colon thus study concludes that hydroalcoholic
extract of Helicteres isora dried fruits have protective effect in acetic acid induced ulcerative colitis.