IN-VITRO ANTI-INFLAMMATORY AND ANTI-ARTHRITIC ACTIVITY OF BARRINGTONIA ACUTANGULA LEAVES

Abstract

The objective of present work is to study the in-vitro anti-inflammatory and anti-arthritic activity of Barringtonia
acutangula leaves, a traditionally used medicinal plant of Madhya Pradesh, India. The ethanolic extract of Barringtonia
acutangula was studied for in-vitro anti-inflammatory activity by human red blood cell (HRBC) membrane stabilization
method and in-vitro anti-arthritic activity by bovine serum protein denaturation method and egg albumin denaturation
method. The activity of ethanolic extract of B. acutangula was compared with standard anti-inflammatory drug
Diclofenec. It is found that B. acutangula extract at concentration of 10, 20, 30, 40 and 50 µg/ml showed 29.95, 43.97,
47.63, 48.66 and 49.69% protection of HRBC in hypotonic solution respectively (IC50: 43.71), whereas, standard
diclofenac at 20, 40, 60, 80 and 100 µg/ml showed 56.28, 60.14, 67.49, 72.78 and 78.69% (IC50: 0.592). In egg
albumin denaturation method, B. acutangula extract at concentration of 10, 20, 30, 40 and 50 µg/ml showed 37.57,
44.16, 60.57, 66.24 and 70.98% inhibition of egg albumin denaturation (IC50 23.36); whereas, standard diclofenac
20,40, 60, 80 and 100 µg/ml showed 47.76, 57.71, 63.89, 75.87 and 84.81% inhibition of egg albumin denaturation
(IC50: 25.31). It was found that ethanolic extract of Barringtonia acutangula was more potent in inhibition of egg albumin
denaturation than diclofenac. It can be concluded that Barringtonia acutangula posses good in-vitro anti-inflammatory and
anti-arthritic activities.