NATIVE BACTERIOCINS AND ITS KILLING ABILITY: A PROMISING APPROACH OF FISH BIOPRESERVATION

Abstract

Fish was assessed for bacteriocin producing LAB [Lactic Acid Bacteria] like bacteria. LAB was screened from intestine of Oreochromis niloticus using Man, Rogosa, and Sharpe (MRS) Lactobacilli agar at 35˚C for 48 h. Antagonistic aspects of bacteriocin were studied by agar well diffusion method against Listeria monocytogenes (ATCC 657, ATCC= American Tissue Type Cell Culture) Aeromonas hydrophila (ATCC 646) and Pseudomonas aeruginosa (ATCC 1688). Bacteriocin substantiality was evaluated at 90˚C temperature, pH 9 and in presence of different enzymes. Thermostable and thermolabile bacteriocins, proteinaceous in nature, capable to inhibit Listeria and Aeromonas victoriously were revealed from fish. Most of the cell associated antimicrobials were reported to be effective against Aeromonas and Pseudomonas. Acidic antimicrobials were functional against Aeromonas and Pseudomonas, whereas hydrogen peroxide as inhibitory compound was successful only against Pseudomonas. Maximum resistances to LAB bacteriocins were communicated by Pseudomonas followed by Aeromonas and least by Listeria. Intrinsic Pediocin like anti-listeria products and undefined thermolabile anti-aeromonas and anti listeria products obtained from research work could be easily used for biopreservation of fish with lest consequences.