PRODUCTION AND APPLICATION OF LACCASE IN BIOTRANSFORMATION OF LIGNOCELLULOSIC MATERIAL AND DECOLOURIZATION OF DYES

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T B Karegoudar
M H Vijayakumar

Abstract

Cladosporium cladosporiodes is a novel strain isolated and evaluated for the production of laccase in submerged
fermentation. Objective of present work was to optimize the laccase production and assessment of delignification in
wheat bran by laccase of Cladosporium cladosporiodes in submerged fermentation. The significant laccase production was
observed in Byred’s medium when grown on wheat bran at static condition. The optimum conditions i.e. pH 5.6,
temperature 40°C, wheat bran 4g, CuSO4
concentration 0.1mM were required for laccase production in submerged
fermentation and there was no any role of tested six aromatic inducers on laccase production. The extracellular laccase
enzyme of Cladosporium cladosporiodes produced from lignocellulosic material fermentation involved in delignification of
wheat bran, biotransformation of lignin model compounds and decolourization eight different azo, metal complex and
triphenyl methane dyes.

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How to Cite
Karegoudar, T., & Vijayakumar, M. (2022). PRODUCTION AND APPLICATION OF LACCASE IN BIOTRANSFORMATION OF LIGNOCELLULOSIC MATERIAL AND DECOLOURIZATION OF DYES. Journal of Advanced Scientific Research, 13(03), 100-106. https://doi.org/10.55218/JASR.202213316
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Research Article