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In the present study a number of bacterial cultures were isolated from environmental soil samples. For this, motor repairs shops are one of the possible site of interest were chosen. Isolated bacterial cultures were further screened for biosurfactant production by using oil displacement method and surface tension measurement. Minimum surface tension reduction value was found 40 dynes/ cm as compared to control (Nutrient broth) 56 dynes/cm. Selected strain also showed β-hemolysis and oil displacement while showed negative result in Cetyltrimethylammonium bromide (CTAB) assay which confirm the nature of biosurfactant. It was found that biosurfactant was not of anionic nature. Identifcation of selected environmental isolate was done using 16s rRNA and was found as Bacillus cereus. Crude biosurfactant extracted with chloroform was found to show antibacterial against methicillin resistant Staphylococcus aureus (MRSA) 43300, Pseudomonas aeruginosa (P. aeruginosa) PAO1, Klebsiella pneumoniae (K. pneumoniae) B5O55 and Salmonella typhi (S. typhi). Thin layer chromatography and autobiography assay to find antimicrobial compound was done.
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