Discovery of Antiproliferative Activity of Novel Bisurea Derivatives Induces Apoptosis in MDA-MB-231 Breast Cancer Cells

Abstract

Breast cancer is the most common type of cancer in women. The prognosis is bleak for triple-negative breast cancer due to the rapid metastasis and ineffective therapeutic options. Finding and developing new medications to treat breast cancer immediately is of the utmost importance. Herein, we investigated the potential mechanisms of bisurea derivatives of p-xylylene diamine cytotoxic activity against breast cancer cells. MTT and microscopic tests were used to measure cell death and cell growth, respectively. It was found out what effects bisurea derivatives of p-xylylene diamine have on caspases. Annexin V/PI staining and cell Analyzer observation of nuclear pieces were used to find cells that had gone into apoptosis. The compound 3c suppressed cell proliferation in the MDA-MB-231 breast cancer cell lines but did not affect normal mammary cells. Additionally, the caspase activities were induced in a higher range in the MDA-MB-231 breast cancer cell lines. Treatment with bisurea derivatives of p-xylylene diamine increased the number of apoptotic cells and led to nuclear pyknosis, fragmentation, and apoptotic body formation in breast cancer cells. The results conclude taken together. Our results suggest that bisurea derivatives of p-xylylene diamine induce apoptosis by activating caspases in MDA-MB-231 breast cancer cells. Moreover, bisurea derivatives of p-xylylene diamine may be an effective strategy for the treatment of breast cancer.