IN-VITRO ANTIMICROBIAL ACTIVITY OF ETHYL ACETATE EXTRACT OF CENTAUREA BEHEN LINN

Abstract

This work aims to evaluate the antimicrobial potential of ethyl acetate extracts of Centaurea Behen Linn (C. Behen) on some microorganisms. Agar well diffusion method has been used to determine the antimicrobial activities of plant extracts against Gram-positive bacteria, Gram-negative bacteria (Streptococcus mutans, Staphylococcus aureus, Salmonella bongori and Escherichia coli), and two fungus (Candida albicans and Aspergillus niger). The extracts exhibited both antibacterial and antifungal activities against tested microorganisms using standard ofloxacin, ciprofloxacin and fluconazole (10-30μg/ml). The antimicrobial activity was determined by measuring the diameter of the zone of inhibition in term of millimeter (mm). To analyze the antimicrobial activity, ethyl acetate extract of C. Behen was tested by well diffusion method against six selected strain and which shows significant inhibitory action against all the tested strain. The antimicrobial activity of ethyl acetate extract of root against all microorganisms was concentration dependent but less than standard drug. It is concluded that the antimicrobial activity showed by the plant is due to the presence of phytochemicals. For future studies, phytochemcials responsible for these activities can be isolated and modified for pharmacological purpose.