STATISTICAL OPTIMIZATION FOR LIPASE PRODUCTION BY PENICILLIUM DIGITATUM OBTAINED FROM DAIRY EFFLUENT

Abstract

Lipases (triacylglycerol acylhydrolases, EC 3.1.1.3) are one of the most important classes of industrial enzymes with an exponential increase in its application in various fields. The active and stable nature of microbial enzymes and the inability of the plant and animal lipases to meet current world demands have been paid more attention in the production of lipase from microbes than from plants and animals. In the present study, the lipase production by Penicillium digitatum isolated from diary waste water was optimized by employing the Box-Behnken design of Response Surface Methodology (RSM). The lipase activity obtained from the experiments was very close to the response predicted by the regression model, which proves the validity of the model. RSM design yielded a maximum activity of 81.77μmol at optimum levels of significant variables of temperature (40˚C) and pH (7.0) after 24 hrs incubation.