DISTRIBUTION OF L-ASPARAGINASE PRODUCING ENDOPHYTIC BACTERIA IN MEDICINAL PLANTS
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Abstract
Microbial endophytes are novel sources of natural bioactive compounds. The present study enlightened endophytes that
produce the anticancer enzyme L-asparaginase, to exploit their potential for mass production. Five plants with anticancer
properties; Catharanthus roseus, Ocimum sanctum, Aloe vera, Withania somnifera and Murraya koenigii were selected as host
plants. Primary screening of L-asparaginase producing bacterial endophytes was done with plate assay method using
phenol red indicator, as a result of hydrolysis of asparagine into aspartic acid and ammonia. Aloe vera plant showed
maximum colonization frequency but C. roseus with highest isolation rate for bacterial endophytes. Results revealed that
a total of 73 bacterial endophytes were isolated. Only 25 of these were able to produce L-asparaginase enzyme, mostly
from C. roseus (08), M. koenigii (05) and W. somnifera (05) followed by O. sanctum (04) and Aloe vera (03). The three
efficient L-asparaginase producing bacterial endophytes were selected through secondary screening and also statistically
proved to be efficient with Tukeys HSD test.
produce the anticancer enzyme L-asparaginase, to exploit their potential for mass production. Five plants with anticancer
properties; Catharanthus roseus, Ocimum sanctum, Aloe vera, Withania somnifera and Murraya koenigii were selected as host
plants. Primary screening of L-asparaginase producing bacterial endophytes was done with plate assay method using
phenol red indicator, as a result of hydrolysis of asparagine into aspartic acid and ammonia. Aloe vera plant showed
maximum colonization frequency but C. roseus with highest isolation rate for bacterial endophytes. Results revealed that
a total of 73 bacterial endophytes were isolated. Only 25 of these were able to produce L-asparaginase enzyme, mostly
from C. roseus (08), M. koenigii (05) and W. somnifera (05) followed by O. sanctum (04) and Aloe vera (03). The three
efficient L-asparaginase producing bacterial endophytes were selected through secondary screening and also statistically
proved to be efficient with Tukeys HSD test.
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How to Cite
Joshi, R., & Kulkarni, N. (2020). DISTRIBUTION OF L-ASPARAGINASE PRODUCING ENDOPHYTIC BACTERIA IN MEDICINAL PLANTS. Journal of Advanced Scientific Research, 11(Suppl 5), 25-31. https://doi.org/2
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Research Article
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