ANTIMICROBIAL ACTIVITY OF RHIZOSPHERIC BACTERIA OF AZADIRACHTA INDICA AND MORINGA OLEIFERA PRODUCING SECONDARY METABOLITES AGAINST HUMAN BACTERIAL PATHOGENS

Abstract

The current study is carried out by the separation and description of Rhizospheric area micro flora and examination of
secondary metabolites against four bacterial pathogens (E. coli, S. mutans, L. acidophilus and S. aureus). Azadirachta indica
and Moringa oleifera plant regions were used for isolation of microbes. The secondary metabolites are not involved in the
growth of the bacterial cultures but they will be resultant of the primary metabolites. Accordingly, after isolation, the
antimicrobial sensitivity study revealed the activity of isolates and further all were categorized through Bergey‟s manual,
the isolates were Sporosarcina, Streptococcus, Micrococcus luteus, Lactobacillus fermentum. It was relatively observed that the
organic solvent had increased the action of the secondary metabolites of the all the effective isolates by many times. The
maximum action found in the Rhizospheric area of Moringa oleifera plant and showed zone of inhibition of 36.5mm against
S. aureus after behavior with the organic solvent methanol and chloroform for intracellular and extracellular secondary
metabolites respectively.