ISOLATION AND MOLECULAR CHARACTERISATION OF MARINE LIPOLYTIC BACTERIA AND STUDY ON EFFECT OF VARIOUS GROWTH PARAMETERS ON LIPASE PRODUCTION

Abstract

Wide variety of industrially important enzymes can be produced by microbes and its significance increases day by day. Microbial lipases are one of such enzyme and high in demand due to its various industrial applications. Marine environment is a vast resource of enzyme producing microbes. The present study aimed to isolate lipase producing bacteria from marine water and identification of hyperactive strain by morphological, biochemical and molecular characteristics. Screening for lipase production were conducted using tributyrin agar and four strains showed a zone of activity. Hyperactive strain was selected by using spectrophotometric assay. Morphological and biochemical characterisation of the selected strain were done and the strain was confirmed as Bacillus kochi by 16s rRNA gene sequence analysis. Under submerged fermentation conditions lipase production found higher at temperature of 37°C with pH 8. Enhanced lipase activity was observed in the presence of sunflower oil and peptone in the production medium when compared with other inducer oils and nitrogen sources.