DEVELOPMENT OF A VALIDATED HPLC METHOD FOR DETERMINATION OF THE FLAVONOID BAICALIN

Abstract

This paper reports a simple validated reversed phase high-performance liquid chromatography method which is fast, precise, and highly specific for determination of baicalin. The separation was achieved using thermo scientific C18 ODS Hypersil column (mm) (250x4.6 mm, 5μm) with a mobile phase consisting of methanol to acetic acid (0.2%) at ratio 94:6, a flow rate of 1.0 ml per minute, and detection at a wavelength of 279 nm. The calibration curve was linear, having a correlation coefficient 0.9997 within range of 2.5–100 μg/ml. The retention time for baicalin was less than 4 minutes. Validation was done according to ICH guidelines with respect to accuracy, linearity, specificity, precision, limit of detection and limit of quantification and satisfactory results were obtained.