STUDIES ON PROTEIN PROFILE OF DIFFERENT SPECIES OF ARBUSCULAR MYCORRHIZAL FUNGI

Abstract

The aim of this study was to investigate the protein profile of four different species of arbuscular mycorrhizal fungi namely, Gigaspora margarita, Glomus mosseae, Glomus fasciculatum and Glomus leptoticum. The mycorrhizal spores were isolated from different location in Chennai and multiplied by trap culture. Allium cepa was used as host plant in trap culture. The Proteins were extracted from AMF associated Allium cepa roots at the time interval of 15 days, 30 days and 45 days. Proteins were estimated using Bradford method and subjected to Sodium Dodecyl Sulphate- Polyacrylamide Gel (SDS PAGE) analysis. Protein profile of mycorrhizal plants reported more number of SDS PAGE bands when compared with non mycorrhizal control plant.