BIODEGRADATION OF PESTICIDE DEGRADING MICROORGANISMS AND IT’S IMPACT

Abstract

ABSTRACT The present investigation was focused on biodegradation of chlorinated pesticide monocrotophos. The enrichment culture technique was adopted in such a way that the high concentrations of 500ppm of pesticides were treated with Paddy field soil for a period of 15 days. Serial dilution technique was employed to isolate the dominant colony with the potential to degrade pesticide. The potential organisms were isolated and characterized morphologically and biochemically. The isolate 1 was presumed to be Bacillus cereus characterized based on morphologically and biochemically. The flask culture technique of biodegradation studies were performed with different concentration of monocrotophos pesticide and it was found out that in higher concentration the degradation was found to be increased. HPLC analysis,revealed a prominent result between control and experimental treated samples. The intermediate metabolites due to the action of enzymatic cleavage in the appearance of splitting isomer entities and breakdown of compounds were formed from Bacillus cereus (S1).