ISOLATION, GENETICAL IDENTIFICATION AND ANTIBIOTIC SENSITIVITY OF SALMONELLA TYPHIISOLATED FROM MARINE EDIBLE FISH RASTRELLIGER KANAGURTA-A NOVEL STUDY

Abstract

Lack of information in view of presence of pathogenic salmonella species in shoaling and schooling marine edible fishes shall cause major adverse health consequences on public health across the globe. In the current study, out of 72 types ofmarine fishes, 12 edible fishes are identified for presence of pathogens including Salmonella. Numerous hazardous microorganisms are isolated from different body parts of fish. Biochemical characterization, 16S rRNA studies were performed for Salmonella species and confirmed the presence of Salmonella typhi. Optimization of media components for the growth of salmonella typhi was performed and genetic identification tests were carried out. Pathogenic evaluation was performed by O-acetyl content and Vi polysaccharide. Antibiotic sensitivity was performed with wide range of antibiotics and concludes that Salmonella typhi threshold levels in view of tolerance of antibiotics i.e., Amoxyclav, Ceftriaxone, Cefotaxime, Cefepime and Aztreonam at a concentration of 20 µg/ml and highly sensitive at concentration of 30 µg/ml per 4 kg body mass index (BMI) of fish. Salmonella typhi threshold levels in view of tolerance of antibiotics i.e., Meropenem, Imipenem, Cefixime, Oxacillin and Ofloxacin at a concentration of 30 µg/ml and sensitive at concentration of 40 µg/ml per 4 kg BMI of fish. For the first time, Salmonella species was isolated from marine edible fish from coastal region, Krishna district, Andhra Pradesh, India and its antibiotic sensitivity was performed. Further studies aims towards vaccine industry in view of public health.