IN-VIVO BIOAVAILABILITY STUDY OF TELMISARTAN COMPLEX IN WISTAR RATS

Abstract

The main of the present study is to evaluate In-Vivo Bioavailability Study of Telmisartan Complex in Wistar Rats. During study periods, rats were housed singly in polypropylene and stainless steel cages. Two to three groups of rats form optimized formulation and one group of rats for marketed formulation were taken. A blood sample was collected from the retro-orbital plexus of anesthetized rats with diethyl ether. For zero time analysis, a blood sample was withdrawn before administration of the drug and after administration, blood samples were collected at the specific intervals. A HighPerformance Liquid Chromatographic System (HPLC) equipped with Shimadzu LC SOLUTION was employed for the present investigation. The system consisted of Shimadzu UFLC 20-AD as a binary solvent delivery system, Shimadzu 7D Rheodyne injector loop injector and Photo Diode Array (PDA) detector as a source of detection. Telmisartan (BCS Class II drug) is an angiotensin II receptor antagonist and these are used in the treatment of hypertension and these are practically insoluble in water. The preliminary studies conducted with quercetin complexes showed that the complex prepared with kneading method and having the drug: complexing agent 1:3 ratios in case of Telmisartan showed better in vitro dissolution rate and hence the resulting complex bioavailability was compared with the pure drug. Pharmacokinetic parameters such as absorption rate constant, biological half life, AUC 0-∞ were calculated for pure drug and bioenhancers complexes. These parameters were treated statistically and significant differences in absorption related terms such as extent of absorption (AUC) and rate of absorption (ka) were noticed.