CHEMICAL CONSTITUENTS ISOLATED FROM SEMECARPUS ANACARDIUM LEAVES 8 ANALYSIS OF THEIR BIO-ACTIVE POTENTIAL

Abstract

Herbal drugs standardization is the need of the time as the use and practice of traditional herbal drugs and their formulations has increased vastly. In the present study, an attempt has been made to isolate the potential bioactives of Semecarpus anacardium as per pharmacognostic testing protocol which include powder microscopy, physico-chemical screening, HPTLC fingerprinting NMR 8 LC-MS analysis. Preliminary phytochemical tests indicate the presence of flavonoids, alkaloids, glycoside, phenols, steroids, protein, sugars and tannins. HPTLC profiling of the ethanol extract using Ethyl Acetate: Water: Formic Acid: Acetic acid (100:26:11:11) v/v/v/v) as mobile phase revealed the presence of phytochemicals with different Rf values. Upon further purification LC-MS 8 NMR analysis of the ethyl acetate fraction showed the presence of semecarpetin as bioflavonoids