PHYTOCHEMICAL SCREENING, ANTIOXIDANT ACTIVITY AND ANTI-BACTERIAL ACTIVITY OF HYDRO-ETHANOLIC EXTRACT OF CAMELLIA SINENSIS LEAVES (HECsL)

Abstract

Recent evolution to find out drugs from natural sources has resulted in compounds that are being developed to treat cancer, resistant bacteria 8 viruses and immunosuppressive disorders. There is growing interest in relationship of the phytochemical constituents of a medicinal plant with pharmacological activity. In this study, phytochemical, antioxidant activity, total phenolic content and concentration of flavonoids of Hydro-ethanolic extract of Camellia sinensis (HECsL) were determined using spectrophotometric methods. HECsL was found to have alkaloids, phenols, flavonoids, tannin,glycosides, saponin, terpenoides, steroids and phytosteroids. The antioxidant activity of extract was expressed aspercentage of DPPH radicals inhibition and IC50 values (μg/ml). Values in percentage ranged to 86.37%. The totalphenolic content ranged to 3.22 mg/g of HECsL extract, expressed as gallic acid equivalents. The total flavonoid concentration was 16.45 mg/g, expressed as gallic acid equivalents. The HECsL extract were evaluated for their antibacterial activity against four Gram-negative and Gram-positive bacteria using ampicillin as positive control. The study revealed that HECsL respective of their types in different concentrations inhibited growth of the test org anism to varying degrees and showed maximum activity against four the bacterial strains. Expression to these results it may be concluded that HECsL extract, regarded as promising candidates for natural plant sources of antioxidants with high value and curing of various infectious diseases caused by the resistant microbes.