IDENTIFICATION OF BIOACTIVE COMPOUNDS EXTRACTED FROM BARKS OF KANDELIA RHEEDIIA AND EVALUATION OF IN VITRO ANTIDIABETIC POTENTIAL

Abstract

The present investigation was aimed to analyze the bioactive compounds of a therapeutically effective plant Kandelia rheedii bark and evaluation of antidiabetic potential by using in vitro assays. By soxhlet extraction, bioactive compounds were extracted followed by evaluation of physical characteristics, percentage extractive value, phytochemical screening and GC-MS analysis of methanolic extract of Kandelia rheedii and methanolic extract was evaluated for antidiabetic activity by employing standard in vitro techniques. The analysis provided seven different compounds like Phenol, 2, 4-bis (1,1-dimethyl ethyl)- (21.4%), Flavone (100%), 4H-1-benzopyran-4-one,7-hydroxy-2phenyl (25.2%), 4H-1 Benzopyram-4-one, 7-hydroxy-2-phenyl (23.8%), 5,8 Octadecadienoicacid, methyl ester-(58.5%), Coumarine, 3-(2,4- dinitrophenyl) (93.8%) and Elaidic acid, isopropyl ester (26.4%). In vitro assays confirm that methanolic extract of Kandelia rheedii exhibited antidiabetic activity. Phytochemical screening and GC/MS analysis can help to implement quantitative estimation with the help of markers and can be helpful for pharmaceutical applications. This study provides scientific evidence that Kandelia rheedii have antidiabetic efficacy.