SCREENINGS OF DIFFERENT EXTRACTIONS FOR ANTIOXIDANT AND HEPATOPROTECTIVE ACTIVITY OF BAUHINIA VARIEGATE LEAF AGAINST PARACETAMOL INDUCED LIVER TOXICITY
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Abstract
The aim of present study was to investigate the effects of leaf extracts of Bauhinia variegate for the treatment of liver
injury induced by the paracetamol. Dried and powdered materials of Bauhinia variegate leaf was extracted with ethanol
and were subjected to phytochemical screening, later partitioned successively to obtain hexane, ethyl acetate and nbutanol partitions, respectively. All partitions were subjected to total phenolic content, total flavonoid content, in vitro
antioxidant studies and hepatoprotective analysis. Following the hepatic injury induction, blood samples and liver were
collected for the respective biochemical parameter and histopathological studies. Our results showed that ethyl acetate
and n-butanol fractions exhibited a strong antioxidant activity in vitro. Ethyl acetate and n-butanol fractions scavenged
DPPH radicals in a dose-dependent manner. In vivo histopathological studies indicated that paracetamol-induced liver
injury was alleviated following ethyl acetate and n-butanol fractions treatment in rats. Ethyl acetate and n-butanol
fractions significantly prevented paracetamol-induced elevations of alkaline phosphatase, glutamate pyruvate
transaminase, aspartate aminotransferase, and total bilirubin levels in rats (P less than 0.05). Moreove r, isolated fractions of
Bauhinia variegate leaf restored the decreased activities of hepatic antioxidant enzymes, including superoxide dismutase,
catalase, and glutathione peroxidase, as well as non-enzyme antioxidant glutathione, which were induced by paracetamol
treatment. From these results it can be concluded that the Bauhinia variegate leaves extracts contain remarkable amount of
flavonoids and can be used as reducing oxidative stress and hepatoprotective agent.
injury induced by the paracetamol. Dried and powdered materials of Bauhinia variegate leaf was extracted with ethanol
and were subjected to phytochemical screening, later partitioned successively to obtain hexane, ethyl acetate and nbutanol partitions, respectively. All partitions were subjected to total phenolic content, total flavonoid content, in vitro
antioxidant studies and hepatoprotective analysis. Following the hepatic injury induction, blood samples and liver were
collected for the respective biochemical parameter and histopathological studies. Our results showed that ethyl acetate
and n-butanol fractions exhibited a strong antioxidant activity in vitro. Ethyl acetate and n-butanol fractions scavenged
DPPH radicals in a dose-dependent manner. In vivo histopathological studies indicated that paracetamol-induced liver
injury was alleviated following ethyl acetate and n-butanol fractions treatment in rats. Ethyl acetate and n-butanol
fractions significantly prevented paracetamol-induced elevations of alkaline phosphatase, glutamate pyruvate
transaminase, aspartate aminotransferase, and total bilirubin levels in rats (P less than 0.05). Moreove r, isolated fractions of
Bauhinia variegate leaf restored the decreased activities of hepatic antioxidant enzymes, including superoxide dismutase,
catalase, and glutathione peroxidase, as well as non-enzyme antioxidant glutathione, which were induced by paracetamol
treatment. From these results it can be concluded that the Bauhinia variegate leaves extracts contain remarkable amount of
flavonoids and can be used as reducing oxidative stress and hepatoprotective agent.
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How to Cite
Chourasia, A., & Soni, L. (2021). SCREENINGS OF DIFFERENT EXTRACTIONS FOR ANTIOXIDANT AND HEPATOPROTECTIVE ACTIVITY OF BAUHINIA VARIEGATE LEAF AGAINST PARACETAMOL INDUCED LIVER TOXICITY. Journal of Advanced Scientific Research, 12(01 Suppl 1), 108-113. https://doi.org/16
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Research Article
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