PREPARATION, STANDARDIZATION AND EVALUATION OF PRELIMINARY ANTI-INFLAMMATORY ACTIVITY OF HERBAL FORMULATION OF CITRULLUS COLOCYNTHIS

Abstract

Present study involves preparation and standardization of topical herbal cream followed by evaluation of antiinflammatory potential of active extract using albumin denaturation method. Topical herbal cream formulation was
prepared using extracts of Citrullus colocynthis. The plant is traditionally known for its anti-arthritic potential therefore
selected for the study. Crude raw material of plant was collected, dried, grinded and sieved through 100 mesh sizes. The
physiochemical constants like extractive values and ash values were determined followed by preparation of plant extract
using maceration method. Formulation was prepared using typical ingredient required for topical formulation. The
standardization of plant constituent was performed using modern analytical technique that after inhibition of bovine
serum albumin denaturation method was utilized to perform anti-inflammatory activity. Study observed appreciable antiinflammatory activity of test compound comparable to the standard drug Diclofenac sodium. The results of study
suggested future possibility of anti-inflammatory topical formulation of Citrullus colocynthis.