HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF EFAVIRENZ, EMTRICITABINE AND TENOFOVIR IN COMBINED DOSAGE FORM

Abstract

This paper describes the development and validation of a HPLC method for the quantization of Emtricitabine, Tenofovir,
and Efavirenz in combined pharmaceutical formulations. The thermo C18 (250 × 4.6 mm, 5 μm) column was used. UV
detection was performed at 254 nm. The mobile phase consisted of 10mM KH2PO4
: Methanol (pH 3.0 with OPA) in the
ratio of 20:80v/v, at the flow rate was 1.0 ml/min in ambient temperature. The injection volume of sample was 20 μl.
The validity and reliability of proposed methods were assessed by recovery studies. The recovery of added standards (80
%, 100 % and 120 %) was found at three replicate and three concentrations level. The value of % means just close to
100, SD and % RSD are less than two, indicate the accuracy of method. The Simplicity, Rapidly and Reproducibility of
the proposed method completely fulfill the objective of this research work.