ISOLATION OF ANTAGONISTIC ACTINOMYCETES FROM MUTHUPET MANGROVE FOREST SOIL AND THEIR ANTIBACTERIAL ACTIVITY

Abstract

The development of new antimicrobial agents, preferably naturally occurring ones with novel mechanisms of action, is an urgent medical need. Soil in particular is an intensively exploited ecological niche, the inhabitants of which produce many useful biologically active natural products, including clinically important antibiotics. In the present study, actinomycetes species were isolated from Muthupet mangrove forest soil samples and analyzed their antagonistic effect. The positive antagonistic actinomycetes colony was identified and antibacterial activity of positive actinomycetes strains was analyzed against antibiotic resistant bacterial strains. Actinomycetes are prolific producers of antibiotics and important suppliers to the pharmaceutical industry, can produce a wide variety of secondary metabolites. Streptomyces parvulus fermentation antibiotic compound was extracted using ethyl acetate by centrifugation and antibacterial compound containing disc were prepared separately, the disc were used for assay of antibacterial activity against antibiotic resistant bacterial strains. Finally concluded that the isolated actinomycetes Streptomyces parvulus was highly recommended for antibiotic production in industrial level, it will create new sector in the pharmaceutical field.