IN-VITRO ANTI-INFLAMMATORY, ANTI-ARTHRITIC AND ANTI-OXIDANT ACTIVITY OF BROMELAIN SUPPLEMENT

Abstract

Bromelain is a complex mixture of proteinases derived from pineapple stems and fruit. The objective of the present work is to study the in-vitro anti-inflammatory, anti-arthritic activity and antioxidant activity of commercially available bromelain supplements. Bromelain was studied for in-vitro anti-inflammatory activity by human red blood cell (HRBC) membrane stabilization method and in-vitro anti-arthritic activity by bovine serum protein denaturation method and egg albumin denaturation method. The activity of bromelain was compared with standard anti-inflammatory drug Diclofenac. Antioxidant activity was determined by the method of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) with vitamin C as a standard. It was found that bromelain showed protection of HRBC in hypotonic solution at IC50 43.71µg/ml, whereas, standard diclofenac showed, IC50 0.592µg/ml. In egg albumin denaturation method, that bromelain inhibition of egg albumin denaturation found IC50 23.36µg/ml; whereas, standard diclofenac showed inhibition of egg albumin denaturation at IC50 25.31µg/ml). Bromelain product showed good antioxidant activity (IC50 28.37µg/ml), as compared with the standard (IC5010.56µg/ml). It was found that bromelain was more potent in inhibition of egg albumin denaturation compared with standard. Finally, from results it can be concluded that bromelain possesses good in-vitro antiinflammatory, anti-arthritic and anti-oxidant activities.