ESTIMATION OF TOTAL PHENOL, FLAVONOIDS AND ALKALOID CONTENT AND EVALUATION OF ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF TINOSPORA CRISPA LEAVES AND FLOWER EXTRACTS

Abstract

Antioxidant and antimicrobial activity of a hydroalcoholic extract of Tinospora crispa leaves and flower were evaluated in the current study. Total phenol, flavonoids and alkaloid content was estimated following standard procedures. Antioxidant activity was assessed by using 2, 2- diphenyl-1-picryl-hydrazyl (DPPH) assay using ascorbic acid as standard antioxidant and antimicrobial activity was calculated by the disc diffusion method. The total phenolic content in leaves and flower extract was found to be 3.600 and 2.044 in mg/g equivalent to gallic acid. The total flavonoid content in leaves and flower extract was found to be 2.491 mg/g and 1.258 mg/g, respectively, equivalent to quercetin. The total alkaloid content in leaves and flower extract was found to be 3.228 mg/g and 0.847 mg/g, respectively, in equivalent to atropine. IC50 for standard ascorbic acid was found to be 17.68μg/ml and for hydroalcoholic extract of leaves and flower was found to be 57.17μg/ml and 94.13 μg/ml, respectively. Antimicrobial activity results suggest that Tinospora crispa is a potential source of broad-spectrum antimicrobial agents. Tinospora crispa leaves and flowers is potential sources of antioxidant and antimicrobial agents and could be used as a natural antioxidant.