SIMPLE AND RAPID RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF PALONOSETRON AND NETUPITANT IN SPIKED HUMAN PLASMA

Abstract

Using a high performance liquid chromatography (HPLC), a meticulous, liable and hasty method was developed for the determination of Netupitant (NTPT) and Palonosetron (PLST) simultaneously. Prior to RP-HPLC, the method employs proteins precipitation as sample preparation with acetonitrile only. Waters C18 column was used to chromatograph the analytes at ambient temperature with isocratic elution of ammonium acetate (pH 6.0)-methanol (35:65, v/v) with flow rate of 1ml/min and UV detection at 229 nm. The chromatographic run time was 10 min for the mixture with retention time of 4.91 min for PLST and 3.16 min for NTPT. Over the range of 250-3500ng/ml, the calibration curves were linear. The method was validated in terms of accuracy, precision, absolute recovery, freeze-thaw stability, bench-top stability and re-injection reproducibility. The within- and between-day accuracy and precision were found to be within acceptable limits less than 15%. The analytes were stable after three freeze-thaw cycles (deviation less than 15%). The planned method was definite for the simultaneous determination of NTPT and PLST in human plasma.