Antibacterial Effect of Green Synthesized Silver Nanoparticles Against Vibrio sp. Isolated from Broiler Chicken

Abstract

In the present study, Ocimum sanctum plant leaf extract was used for extracellular synthesis of metallic silver nanoparticles (AgNps) to investigate antibacterial activity against Vibrio sp. isolated from broiler chicken. Stable AgNps were formed after adding aqueous solution of 0.75 mM AgNO3 with the O. sanctum plant leaf extract. Quantitative analysis of green synthesized AgNps was monitored by UV-visible spectroscopy. The size and shape was characterised by atomic force microscopy (AFM). In UV-visible spectroscopy, the peak length was reduced within three hours and the average size of the nanoparticles ranged from 10 to 15 nm was observed in the AFM. Bacteriological test were performed in luria-bertani (LB) medium on solid agar plates supplemented with different concentration of 100 to 400 μl green synthesized AgNps. Among the various concentration, 300 μl and 400 μl of nanoparticles showed higher inhibitory activity against the Vibrio sp. The present study clearly indicated that the green synthesized AgNps has potent antibacterial activity against Vibrio sp. O. sanctum based AgNps may be suitable for the formulation of new types of bactericidal activity and which can be effectively used in poultry farms for treating the Vibrio disease.