UNVEILING THE PHYTOCHEMICAL PROFILE, SECONDARY METABOLITE QUANTIFICATION AND ANTIOXIDANT ACTIVITY OF CLEMATIS WIGHTIANA WALL. EX WIGHT and ARN.

Abstract

Traditionally, the leaves of Clematis wightiana have been used in the treatment of rheumatism, indigestion, headaches, varicose veins, bone problems, nasal congestion and sinus. The present study was conducted to evaluate the
phytochemical profile, quantification of secondary metabolites and free radical scavenging capacity of C. wightiana leaf.
The total phenolic, tannin and flavanoid content of C. wightiana leaves were quantified and were found to be higher in the
ethyl acetate extract. Subsequently, the extracts were subjected to appraise their antioxidant capacity by availing various
in vitro antioxidant assays namely DPPH radical scavenging assay, ABTS assay, Phosphomolybedenum assay, Ferric
Reducing assay, Superoxide Radical Scavenging assay and Reducing power assay. The results of the antioxidant assays
revealed that the ethyl acetate extract of C. wightiana leaves possess better free radical scavenging activity than other
solvent extracts. Thus, the finding of the study elucidates the perception on phytochemical and bioactivity of C. wightiana
which could be used in development of phytotherapeutics to enchance human health.
Keywords: Clematis wightiana, Antioxidant, Superoxide radical scavenging, In vitro antioxidant assay, DPPH,
Ethnomedicine.