VALIDATED UV- SPECTROPHOTOMETRIC METHODS FOR THE SIMULTANEOUS ESTIMATION OF DEXTROMETHORPHAN AND CETRIZINE

Abstract

Using UV Spectrophotometric methods, a simple, precise and accurate approach is devised and verified for the
simultaneous assessment of Dextromethorphan (DEX) and Cetrizine (CET) in combined dosage form. The approach 1 is
the Dual wavelength method, which was developed, evaluates the difference in the absorbance of mixtures at wavelengths
where single drugs have the same absorbance and vice versa. Approach 2 is a mean-centered ratio method that relies on
the production of calibration by dividing the spectra of one substance by the spectra of the other. Approach 3 is a
derivative spectrum method that relies on the derivative spectrum's zero crossing points to create calibration for two
medications in the presence of the second. Simultaneous estimation of DEX and CET using the Q-absorption method and
the simultaneous equation method has already been published. The two techniques Simultaneous equation method
(Approach 4) and Q-Absorption method (Approach 5) are repeated to preserve the same circumstances throughout the
experiment. To assess the accuracy and precision of all five approaches, six replication tests and recovery investigations
utilizing recognized synthetic blends are used. The calibrations are used to analyze two pharmaceuticals contained in the
tablet. The procedures have been validated in accordance with ICH recommendations.