MEDIUM FORMULATION AND OPTIMIZATION OF CULTURE CONDITIONS TO MAXIMIZE PROTEASE PRODUCTION BY NOCARDIA SP. LCJ15A
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Abstract
The objective of this study was to optimize the production medium for Nocardia sp. LCJ15A, isolated from the soil
samples of Pichavaram mangroves, Tamil Nadu which showed high yields of protease activity. The isolate Nocardia sp.
LCJ15A was studied in 6 separate basal media for selection of the best medium. Subsequently protease production by
Nocardia sp. LCJ15A was further optimized by submerged fermentation. Various physical and nutritional parameters
were optimized for increasing the production of protease. Optimized parameters included carbon, nitrogen sources,
inducers and their concentration, pH and temperature. Plackett-Burman design assisted in finding the important
parameters which showed a strong influence on protease production by Nocardia sp. LCJ15A. Maltose (A), casein (B),
pH (C) and temperature (D) were selected as model factor for the design. A full factorial design was used to study the
effects of carbon and nitrogen sources in protease production by Nocardia sp. LCJ15A. The 4 main factors were evaluated
by one factor at a time (OFAT) method. This statistical optimization method led to production of 350.99±1.8 U/mL of
protease which was 3.5 fold greater when compared to unoptimized medium. Analysis of variance (ANOVA) revealed a
high coefficient of determination (R2
) value of 0.9154, confirming a reasonable adjustment of the quadratic model with
the experimental data. Production of improved protease enzyme yields from Nocardia sp. LCJ15A maximizes its
applicability in various industries.
samples of Pichavaram mangroves, Tamil Nadu which showed high yields of protease activity. The isolate Nocardia sp.
LCJ15A was studied in 6 separate basal media for selection of the best medium. Subsequently protease production by
Nocardia sp. LCJ15A was further optimized by submerged fermentation. Various physical and nutritional parameters
were optimized for increasing the production of protease. Optimized parameters included carbon, nitrogen sources,
inducers and their concentration, pH and temperature. Plackett-Burman design assisted in finding the important
parameters which showed a strong influence on protease production by Nocardia sp. LCJ15A. Maltose (A), casein (B),
pH (C) and temperature (D) were selected as model factor for the design. A full factorial design was used to study the
effects of carbon and nitrogen sources in protease production by Nocardia sp. LCJ15A. The 4 main factors were evaluated
by one factor at a time (OFAT) method. This statistical optimization method led to production of 350.99±1.8 U/mL of
protease which was 3.5 fold greater when compared to unoptimized medium. Analysis of variance (ANOVA) revealed a
high coefficient of determination (R2
) value of 0.9154, confirming a reasonable adjustment of the quadratic model with
the experimental data. Production of improved protease enzyme yields from Nocardia sp. LCJ15A maximizes its
applicability in various industries.
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How to Cite
Gnanadoss, J., & Swarna, D. (2020). MEDIUM FORMULATION AND OPTIMIZATION OF CULTURE CONDITIONS TO MAXIMIZE PROTEASE PRODUCTION BY NOCARDIA SP. LCJ15A. Journal of Advanced Scientific Research, 11(Suppl 6), 28-39. https://doi.org/1
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Research Article
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