EFFECT OF AQUEOUS EXTRACT OF TENDER MANGO SEED ON BLOOD COAGULATION, FIBRINO(GENO)LYTIC ACTIVITIES AND ON PLATELET FUNCTION

Abstract

This study high lights on the anticoagulant, fibrino (geno) lytic and antiplatelet activities of Aqueous Extract of raw
Tender Mango Seed (RGMS). RGMS showed varied molecular weight proteins bands from the range 65kDa to 14.3 kDa
on SDS-PAGE under reduced and non-reduced condition in a similar way. When, RGMS was analyzed for its probable
carbohydrate content using PAS-staining, only single protein at the low molecular weight region taken up the PAS stain.
RGMS digested casein with the specific activity of 1.106 units/mg/min at 37˚C. The proteolytic activity was inhibited by
only 1, 10, Phenanthrolene but IAA, PMSF, EDTA and EGTA did not affect the activity, suggesting the presence of zinc
dependent metalloprotease. Furthermore, RGMS showed strong anticoagulant effect in plasma coagulation time by
extending the clotting time from control 250 sec to 1200sec. RGMS hydrolyzed only Aα chain of the fibrinogen without
affecting Bβ and γ chains. In addition, RGMS degraded all the chains of fibrin clot in a dose dependent manner. Above
all, RGMS also exhibited antiplatelet activity by inhibiting agonists ADP and epinephrine induced platelet aggregation of
platelet-rich plasma. The platelet aggregation inhibition was an extent of 80% 96% respectively. Moreover, RGMS was
non toxic to Red Blood Cells. Taken an account of said properties, RGMS could be further exploit in the treatment of
thrombotic disorders.