OPTIMIZATION OF PROCESS PARAMETERS FOR LACCASE PRODUCTION BY SOLID STATE FERMENTATION FROM TRAMETES ELEGANS H6

Abstract

Laccases (E.C. 1.10.3.2) are multinuclear enzymes containing copper and belong to the class of oxidoreductase enzyme.
The main function of laccase enzymes is to catalyse the oxidation of a variety of phenolic and inorganic compounds, with
the consequent reduction of oxygen to water-H2O. The aim of the present study was to obtain the most potent laccase
producer in which after screening a total of 34 fungal strains were obtained from different samples of decayed wood, tree
bark and soil from varying biotopes of Valsad and Vapi region of Gujarat, India. These isolates were subjected to primary
screening for evaluating their phenol oxidase activity. A total of 16 strains exhibited brown color zone on malt extract
agar plate containing orthodianisidine (0.01% w/v). In the secondary screening, Fungal isolate H-6 showed maximum
laccase activity with ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) and identified as working strain in
solid state fermentation . Isolate H6 was identified as a Trametes elegans using ITS sequencing. In the second phase of the
study optimization of the process parameters such as incubation time, moisturing agent, innoculam size, pH,
Temperature, carbon source and nitrogen source were carried out for the hyper production of laccase using Trametes
elegans H6.