ANTIMICROBIAL ACTIVITY OF DIETHYL PTHALATE ISOLATED FROM MARINE STREPTOMYCES MW09-1: AN INSILICO APPROACH

Abstract

Marine actinomyces MW09-1 isolated from coastal area of athirampattinam, Thanjavur, Tamil nadu, India. The strain was identified as stretomyces species based on morphological, physiological, biochemical characters along with 16s rRNA analysis. The active extract was purified and the structure elucidated by GC-MS, NMR Spectra. It was identified as diethyl phthalate. Diethyl phthalate identified from the stretomyces strain showed significant antibacterial activity against uropathogenic E.coli with a zone of inhibition value of 16.6±2.08mm. Insilico Docking analysis was carried out by binding affinities between Diethyl phthalate, Ciproflaxcin and Intimin protein expressed by EAE gene. It showed that the compound had minimum binding energy of -25.3388 kg/ml and had a good affinity. Hence the diethyl phthalate could be an effective natural antimicrobial compound.