IN-VITRO CYTOTOXICITY OF FERMENTED INEDIBLE FRUIT AND VEGETABLE WASTES ON NORMAL FIBROBLAST CELLS AND MCF-7 BREAST CANCER CELLS

Abstract

In recent times, plant derived drugs are being utilized to compete the breast cancer, due to the variety of phytochemicals present in them and also because they have no or lesser adverse effects compared to the chemical drugs. In our study, the inedible wastes obtained from vegetable and fruits (Peel of Allium cepa and Punica granatum; Seed of Syzygium cumini and Mangifera indica) were fermented and their methanolic extracts were prepared. The cytotoxicity of extracts on human breast cancer cell line (MCF-7) and normal fibroblast cell line was studied by MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5- diphenyltetrazolium bromide) assay. All the extracts turned out to be more cytotoxic for MCF-7 cell line and less cytotoxic for normal fibroblast cell line. The fermented methanolic extract of Allium cepa peel is found to be most toxic against MCF-7 cell line. Upon further investigations, these wastes may provide us potent natural anti-breast cancer compounds.