QUANTIFICATION OF PREGABALIN AND ETORICOXIB COMBO IN TABLETS AND BULK WITH DEVELOPED RP-HPLC METHOD: STABILITY INDICATING FEATURE ASSESSMENT

Abstract

This investigation reports a stability indicating HPLC method to quantify Pregabalin (PGBN) and etoricoxib (ERCB) in tablets form and bulk form. The PGBN and ERCB were quantified on Thermo C18 column with Orthophosphoric acid (0.1%), and methanol 60:40 vol/vol ratio was employed as mobile phase. The amounts of PGBN and ERCB were enumerated by detector fixed at 236 nm. The procedure demonstrated reasonable linearity for PGBN with R2 = 0.9998 in the quantity scale 37.5 to 112.5 µg/ml, and for ERCB with R2 = 0.9999 in the quantity scale 30 to 90 µg/ml. The LOD as well as LOQ for PGBN are 0.201µg/ml 6 0.670 µg/ml and for ERCB are 0.106 µg/ml 6 0.355 µg/ml. The RT’s for PGBN was 2.636 min and ERCB was 5.607 min and whole analysis time was 8 min. Stability degradation experiments were used to establish the method's capacity to indicate stability indicating. The conditions include acid forced hydrolysis, thermal forced degradation, alkali forced hydrolysis, oxidative forced degradation and photo forced degradation conditions. Detection as well as quantification of PGBN and ERCB was unaffected by the degradants.