ANTIVIRAL EFFECTS OF L-FUCOSE FROM PADINA GYMNOSPORA AGAINST VARIOUS STRAINS OF DENGUE VIRUS

Abstract

Now a days, dengue is a very serious communal and economic public health crisis which impose continuous search of natural bioactive compounds to manage its control. The main focus of the present study is to explore L-Fucose from marine algae as phytotherapeutics for dengue treatment. Dengue is a major mosquito-borne disease andtill date no efficient antiviral drug or vaccine is available on the market for control of it. The L-Fucose is reported to exhibit antiviral properties but its mechanism on inhibition of dengue virus DENV1-4 was not studied in detail. In this study, the nontoxic concentration of the drug to the normal cell was calculated and its potential antiviral activity against dengue virus DENV1-4 was evaluated using Vero cell line. Experiments were conducted in vitro to determine the potency of polysaccharide against dengue virus by propagating them in Vero cell lines. Viral titer was determined and cytotoxicity assay were performed by CC50. The antiviral potential of the medicine was detected by using MTT assay and Plaque Assay. The co-treatment technique used in antiviral assay with concentration 40µg/ml showed better antiviral activity.