ASSOCIATION OF GENETIC VARIANTS IN THE PROMOTER REGION OF APOC3 WITH THE RISK OF NON-ALCOHOLIC FATTY LIVER DISEASE (NAFLD) IN NORTH INDIAN POPULATION

Abstract

Non-Alcoholic Fatty Liver Disease (NAFLD) is a clinical condition defined by excessive fat accumulation in the liver in the form of triglycerides involving more than 5% of hepatocytes. The present study was carried out with an aim to evaluate the association of demographic features, liver enzymes, serum lipid profile and APOC3 gene variants (C-482T and T-455C). A case-control study was designed with 150 patients with NAFLD, and 180 healthy controls. The mutations were analyzed using PCR-RFLP approach, Case-control studies assessing the relationship between APOC3 rs2854116 C/C and rs 2854117 C/T gene polymorphism with non-alcoholic fatty liver disease. Statistically significant increased frequencies of CC+CT genotype [OR, 2.00; 95 percent C. I (1.18-3.40); P.0006] [OR, 2.00; 9.5 percent C.I (1.38-2.91); P value < 0.0002] and the C allele is seen in NAFLD patients compared to healthy controls. In conclusion, we found that the APOC3 gene and elevated triglyceride levels in -455C/C SNP is significantly associated with the risk of NAFLD, whereas the Apoc3-482C/T showed no association.