RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF BRIVARACETAM IN BULK AND FORMULATION

Abstract

The analytical method was developed and validated for estimation of Brivaracetam in bulk and formulation by Reverse
Phase High performance liquid chromatography. The separation was carried out on Luna C8 (150 × 4.6mm, 5μm)
column. The mobile phase consists of ACN: Water at flow rate 1 ml/min at 208nm. The column temperature was
adjusted at 30°C with injection volume 20μl. The retention time of Brivaracetam was 3.1 min. The linearity of the
calibration curve was linear over the concentration range 10-50 μg/ml. The developed method was validated according
to the International Council for Harmonization (ICH) Guidelines. The developed method was easy, rapid, linear,
precise, accurate and consistent. So, the method can be successfully applied for the routine analysis of Brivaracetam in
pharmaceutical formulation.