COMPARATIVE HEPATOPROTECTIVE ACTIVITY OF AQUEOUS EXTRACT OF TRIPHALA AND SOLID LIPID NANOPARTICLE IN ISONIAZID-RIFAMPIN AND TETRACYCLINE INDUCED HEPATOTOXICITY IN RATS

Abstract

The aim of the present study was to investigate the effect of hepatoprotective activity of aqueous extract of triphala and
solid lipid nanoparticle in isoniazid-rifampin and tetracycline induced hepatotoxicity in rats/mices. Wistar Albino rats
and mice of either sex weighing between (200-250 and 35-40 gm) were divided in seven groups of each containing six
rats/mices and treated for 21 days. Group I and II served as normal and toxic control, Group III were treated with
Silymarin (25 mg/kg), and Group IV to VII were treated with aq. extract of triphala (100mg/kg), solid lipid
nanoparticles of triphala (50mg/kg), gallic acid (50mg/kg) and solid lipid nanoparticles of gallic acid respectively. The
biochemical markers like (AST, ALT, ALP and serum bilirubin) were estimated. The in vivo antioxidant activity was
determined by estimating the tissue levels of glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and lipid
peroxidation (LPO) and histopathology of liver was also carried out. The triphala/ bioactive & their formulation (100
and 50mg/kg) produced significant effect by decreasing the activity or level of AST, ALT, ALP and serum bilirubin and
while it significantly increased the levels of tissue GSH, SOD and CAT in a dose-dependent manner. From the present
study it can be concluded that aqueous extract of triphala/bioactive & their formulation possesses hepatoprotective
activity against isoniazid-rifampin and tetracycline induced hepatotoxicity.