PHYTOCHEMICAL ANALYSIS AND IN VITRO ANTIOXIDANT STUDIES OF LAGERSTROEMIA PARVIFLORA ROXB BARK

Abstract

The objectives of present studies are to screen the phytochemicals, estimate the content of phenolic and flavonoid
compounds and determine the antioxidant capacity of the Lagerstroemia parviflora Roxb (L. parviflora) barks. Qualitative
analysis of various phytochemical constituents and quantitative analysis of total phenol and flavonoids were determined
by the well-known test protocol available in the literature. The hydro alcoholic extract of bark of L. parviflora was studied
for antioxidant activity on different in vitro models namely 1,1-diphenyl, 2-picryl hydrazyl (DPPH) assay, Hydrogen
peroxide and Nitric oxide radical scavenging method. Phytochemical analysis revealed the presence of phenols and
flavonoids. The total phenolic and flavonoids content of L. parviflora bark of hydroalcoholic extract was 0.247and
0.687mg/100mg respectively. Ascorbic acid used as standards was also evaluated for comparison. The extract showed
dose dependent free radical scavenging property in the tested models. L. parviflora bark extract showed IC50 value
60.22μg/ml for DPPH method, which was comparable to that of ascorbic acid (IC50=17.68μg/ml). For hydrogen
peroxide method, IC50 value was found to be 88.60μg/ml, which compares favourable with ascorbic acid
(IC50=36.613μg/ml). In nitric oxide model, IC50 value was found to be 91.73μg/ml, which was comparable to that of
ascorbic acid (IC50=24.63μg/ml). The present study describes the phytochemical profile and antioxidant activity of L.
parviflora which will further used for medicinal applications.