EVALUATION OF ANTICANCER PROPERTY OF AMBLYONE FROM AMORPHOPHALLUS PAEONIIFOLIUS (ELEPHANT FOOT YAM) USING IN SILICO ANALYSIS

Abstract

The present work aims at evaluating the anticancer activity of Amblyone, a phytochemical present in the tuber of
Amorphophallus Paeoniifolius (Elephant foot yam) and compare its activity with the commonly used antiapoptotic drug
Tetrahydroisoquinoline amide substituted phenyl pyrazole derivative. Anti cancer activity is analyzed by docking the
ligands against the antiapoptotic B-Cell Lymphoma-2 protein (Bcl-2) [PDB ID: 2w3l] using AutoDock Tool software. A
docking score of -12.2 kcal/mol is obtained for the phytochemical Amblyone compared with-7.7 kcal/mol for
Tetrahydroisoquinoline amide substituted phenyl pyrazole derivative. The better docking score is an indication that
the amblyone can bind firmly on the groove of Bcl-2 protein which regulates the activity of pro-apoptotic proteins by
direct binding and sequestration. The absorption, distribution, metabolism, excretion (ADME) and toxicity properties of
the ligands are studied using Pre ADMET software. The in silico studies are further supported by in-vitro studies of the
crude sample using A549 (Lung cancer) cells by direct observation of cells using Inverted phase contrast microscope
followed by colorimetric assay method (MTT) and direct microscopic observations.