SOIL BACTERIAL ISOLATES SHOWING PROMISING FIBRINOLYTIC ACTIVITY

Abstract

Directly acting fibrinolytic enzyme is found to be superior therapy for treatment of cardiovascular diseases. Hence, there
is a need of a novel and highly potent fibrinolytic agent which can directly degrade fibrin clot in lesser time with
improved specificity and overcome shortcomings of haemorrhage. Amongst various sources, microbial fibrinolytic
enzymes have attracted attention of research community so the present study aimed to isolate fibrinolytic enzymeproducing
bacteria from different soil samples. Caseinolytic activity and clot lyses activity of the crude enzyme produced
by these isolates were determined. All together six caseinolytic organisms were isolated, 3 of them produced fibrinolytic
protease which would lyse fibrin and whole blood clot. Caseinolytic activity in the crude enzyme preparation from
Colony C₄ was detected to be 78.2 U/ml and was able to lyse 85.54% of whole blood clot in 90 mins. Colony 4 may be
of potential interest for biotechnological use as it produces fibrinolytic proteases that degraded fibrin clot and the whole
blood clot, to the maximum.