DEVELOPMENT OF HPTLC DENSITOMETRIC METHOD FOR ESTIMATION OF QUERCETIN IN BOMBAX CEIBA L. LEAVES

Abstract

Bombax ceiba L. is a medicinally valuable herb in the ayurvedic and traditional systems of medicine. Various activities have been reported in almost all parts of Bombax ceiba, some of these include hypertensive, antioxidant, hypoglycemic, hepatoprotective, and antipyretic. Quercetin, one of the most important flavonoids is active against various cardio vascular diseases, cancer, tuberculosis, neurological diseases, cataract etc. In the present study High Performance Thin Layer Chromatography method has been developed for detection and quantification of quercetin in Bombax ceiba leaves. Increasing serial dilutions of reference standard quercetin (200 to 1000 μg/ml) were scanned at 366 nm to detect and quantify the concentrations of quercetin in the test sample. The estimated value obtained from the same was 5.38% quercetin in powdered leaf sample. The method provided a rapid and easy approach for detection and the quantization of the bio-marker quercetin.