ISOLATION AND IDENTIFICATION OF ANTI-ESBL (EXTENDED SPECTRUM β-LACTAMASE) COMPOUND FROM MARINE STREPTOMYCES SP. VITSJK8

Abstract

Multidrug resistant extended spectrum β-lactamse producing organisms are more frequently reported from urinary tract infected UTI patients. Recent reports have shown that ESBL’s efficiently hydrolyze third generation cephalosporins and monobactams, thereby limiting the therapeutic options. The aim of the present study was to isolate and to identify anti-ESBL compound from marine Streptomyces sp.VITSJK8. It was isolated from marine sediment sample collected at Cheyyur beach, Bay of Bengal, Tamil Nadu, India. The ethyl acetate extract from the culture supernatant of Streptomyces sp.VITSJK8 was prepared and purified by silica gel column chromatography and HPLC. The purified compound was identified by using 1H and 13C NMR spectral data and based on the similarity index with reference compounds available in the mass spectra library of National Institute for Standards and Technology as 1, 2-benzene dicarboxylic acid, mono (2-ethylhexyl) ester. The anti-ESBL activity of the purified compound was tested against ESBL isolates according to the National Committee for Clinical Laboratory Standards guidelines and it was revealed that its MIC50 value ranged from 0.13 to 2.00 μg/mL. The results of the study suggest that Streptomyces sp.VITSJK8 is the potential source for anti-ESBL compounds.