INVESTIGATING THE BINDING INTERACTIONS OF NADOLOL WITH HUMAN SERUM ALBUMIN- A COMBINED THERMODYNAMIC AND MOLECULAR MODELLING APPROACH

Abstract

Nadolol is a beta blocker, commonly used in the cure of arterial hypertension. In order to accomplish an optimal
performance and efficacy of this drug molecule, it is important to consider the possible complexation of nadolol with
human serum albumin. As the degree of interaction of a drug to plasma proteins has a visible effect on its delivery,
elimination and pharmacological effect as only the unbound part of drug is available for distribution into extra-vascular
part. In this work, we have chosen Isothermal titration calorimetry and Molecular docking techniques to investigate the
binding interactions of Nadolol with human serum albumin. The ITC data revealed that the binding between nadolol and
Human Serum Albumin was through entropy driven route and the association constant Ka=8.73×106M−1and the results
obtained by ITC were complemented with in-silico studies.